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A novel diblock copolymer, consisting of a biocompatible phosphorylcholine block (MPC) and a pH sensitive dimethylamino ethyl block (DMA), denoted as MPC30DMA60, shows interesting properties of surface adsorption and solution aggregation. These features have been exploited to condense DNA into nanoaggregates in bulk solution and to immobilise DNA onto a substrate surface. These molecular manipulations...
A novel diblock copolymer, consisting of a biocompatible phosphorylcholine block (MPC) and a pH sensitive dimethylamino ethyl block (DMA), denoted as MPC30DMA60, shows interesting properties of surface adsorption and solution aggregation. These features have been exploited to condense DNA into nanoaggregates in bulk solution and to immobilise DNA onto a substrate surface. These molecular manipulations...
The availability of high quality cDNA libraries is often crucial to the successful identification and characterization of genes. The concepts and potential pitfalls of constructing cDNA libraries are presented. Various applications requiring high quality cDNA libraries are outlined, including large-scale single pass sequencing of cDNA clones to generate expressed sequence tags (ESTs) and differential...
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