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Leuconostoc citreum EFEL2700 isolated from kimchi was used as a host strain for genetic and metabolic engineering in our previous studies, but the cells of EFEL2700 contained a cryptic plasmid (P‐cells). Thus, we created plasmid‐free cells (F‐cells) using the CRISPR/Cas9 system. In this study, we compared the microbial characteristics of P‐ and F‐cells in terms of growth rate, biochemical properties,...
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