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The YeaZ protein of Vibrio harveyi was expressed in Escherichia coli and purified. The purified recombinant protein YeaZ exhibited the protease activity. The proteolytic activities with azocasein as substrate were 39 130 U mg−1. The mutation of the amino acid in active sites such as Asp88, Ser185 and Trp169 was performed. The enzyme activities of the purified mutant proteins with Asp88‐Ala, Ser185...
Aims
To demonstrate the resuscitation‐promoting activities of recombinant YeaZ from Vibrio harveyi SF‐1.
Methods and Results
The gene of resuscitation‐promoting factor YeaZ was cloned from genomic DNA of V. harveyi SF‐1. The gene was expressed in Escherichia coli, and the expressed protein was purified by Ni2+‐affinity chromatography. A yeaZ mutant was constructed by using the suicide plasmid pNQ705...
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