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Three sets of oligonucleotide primers (Vf1 and Vr1, Vf2 and Vr2, Bf and Br) were derived from a D. nodosus virulent-specific clone pV470-13 (2146 bp) and a benign-specific clone pB645-335 (737 bp), respectively. Using the virulent-specific primers Vf1 and Vr1 in a polymerase chain reaction (PCR) enabled amplification of a DNA fragment of 460 bp in 2527 virulent, 925 high intermediate,924 low intermediate...
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