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Genome engineering strategies employing site‐specific recombinases (SSRs) have become invaluable to the study of gene function in model organisms. One such SSR, the integrase encoded by the Streptomyces bacteriophage phiC31, promotes recombination between heterotypic attP and attB sites. In the present study I have examined the feasibility of the use of phiC31 integrase for intramolecular recombination...
Single optical section of a 72 hour post‐fertilization T2K‐XpGbR (EF1‐alpha‐attP‐GFP‐attB‐DsRed‐Express) transgenic zebrafish larva obtained by laser scanning confocal microscopy. Injection of phiC31 integrase messenger RNA at the one‐cell stage induces recombination of the transgene in a mosaic fashion, resulting in excision of the green fluorescent protein cassette and expression of DsRed fluorescent...
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