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The aim of this study was to examine the changes of ATF4 expression in cultured human periodontal ligament fibroblasts (hPDLF) after mechanical stimuli, and to investigate whether ATF4 is essential for the mechanical stress-induced hPDLF differentiation.Reverse transcriptase polymerase chain reaction (RT-PCR) and western blotting were used to examine mRNA and protein levels of ATF4 expression in hPDLFs...
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