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In the presented work, a disposable immunosensor for the detection of testosterone, an endogenous steroid hormone, in bovine urine has been developed using screen-printed electrodes (SPEs). Due to concerns over the use of steroid hormones as growth promoters, the EU prohibits their use in food producing animals. Consequently, rigorous screening procedures have been implemented in all member states...
This work reports on the development of rapid enzyme-linked immunosorbent assay for testosterone, methyltestosterone, 19-nortestosterone and their metabolites in real samples of bovine urine. The assays were based on the competition between an immobilised testosterone–BSA conjugate and the analyte for corresponding antibodies, followed by the use of secondary anti-species (α-IgG-HRP) to determine...
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