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Contrary to batch processes performed in RPMI medium containing 5% serum, CHO cells producing a recombinant IFN-γ and cultivated in fed-batch process with the same medium, were capable to maintain a high energetic status during the first hours of the cultures. Interestingly, IFN-γ was produced mainly during this period and remained highly glycosylated contrary to batch processes. The same phenomenon...
Évaluer la faisabilité et la toxicité aiguë d'une nouvelle technique de curiethérapie interstitielle de prostate par implant permanent d'iode 125, caractérisée par l'utilisation d'un projecteur de sources après dosimétrie tridimensionnelle peropératoire (technique FIRST).Trois cent quatre-vingt-quinze patients ont été traités en France par six centres de radiothérapie entre novembre 2002 et décembre...
Analysis of intracellular nucleotide and nucleotide sugar contents is essential in studying protein glycosylation of mammalian cells. Nucleotides and nucleotide sugars are the donor substrates of glycosyltransferases, and nucleotides are involved in cellular energy metabolism and its regulation. A sensitive and reproducible ion-pair reverse-phase high-performance liquid chromatography (RP-HPLC) method...
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