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Purpose: To know the regulation of tau within cells, tau was phosphorylated by cdk5 kinase with or without microtubules (MT) in vitro.Method: Phosphorylation sites in tau were determined by western blotting. Effects of phosphorylations on MT dynamics were examined by dark-field microscopy and light scattering measurement.Results: Phosphoryl groups were incorporated into both Ser-235 and Ser-404 independent...
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