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We present the environmental-SEM (ESEM)-nanomanipulator system for in-situ measurement of mechanical properties of the yeast cells (W303 strain). This enhanced ESEM system comprises of a standard ESEM instrument as a nano imaging tool, a cooling stage as a cellular biology's humidity controller and 7 D.O.F. linear actuators as nanomanipulator/effector. Two types of experiments have been conducted,...
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