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Background: To gain insight into the local and nonlocal interactions that contribute to the stability of hen lysozyme, we have synthesized two peptides that together comprise the entire α-domain of the protein. One peptide (peptide 1–40) corresponds to the sequence that forms two α-helices, a loop region, and a small β-sheet in the N-terminal region of the native protein. The other (peptide 84–129)...
Non-native states of proteins are of increasing interest because of their relevance to issues such as protein folding, translocation and stability. A framework for interpreting the wealth of experimental data for non-native states emerging from rapid advances in experimental techniques involves comparison with a ‘random coil’ state, which possesses no structure except that inherent in the local interactions...
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