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White blood cells are isolated from whole blood in silicon–glass 4.5-μl microchips containing a series of 3.5-μm feature-sized ‘weir-type‘ filters, formed by an etched silicon dam spanning the flow chamber. Genomic DNA targets, e.g., dystrophin gene, can be directly amplified using the polymerase chain reaction (PCR) from the white cells isolated on the filters. This dual function microchip provides...
Random amplification of the human genome using the degenerate oligonucleotide primed–polymerase chain reaction (DOP-PCR) was performed in a silicon–glass chip. An aliquot of the DOP-PCR amplified genomic DNA was then introduced into another silicon–glass chip for a locus-specific, multiplex PCR of the dystrophin gene exons in order to detect deletions causing Duchenne/Becker muscular dystrophy. Amplicons...
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