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We present a Pulsed Laser Activated Cell Sorter (PLACS) integrated with 3D sheathless inertial focusing that is capable to sort at 10,000 particles sec−1 with >90% sort purity and 6,000 cells sec−1 with >80% sort purity. It is realized by exciting laser induced cavitation bubbles in a single layer PDMS microfluidic channel to create high speed liquid jets to deflect detected fluorescent samples...
We report a 3D PDMS microfluidic pulsed laser triggered fluorescence activated cell sorter capable of sorting at 11,000 cells sec−1 with >95% purity or at 45,000 cells sec−1 with 45% purity within a single channel.
Port Wine Stain (PWS) birthmarks are congenital vascular malformations, which usually appear at birthand tend to become darker and thicker with age. Vascular-targeted Photodynamic Therapy (PDT) is an effective approach among treatments for PWS. However, due to the arbitrariness of manual operation and pole points in laser radiation, the PWS zone was always cured unevenly in clinics. This paper presents...
We report a high speed microfluidic fluorescence activated cell sorter (μFACS) capable of sorting at a throughput of 3000 beads/sec and 560 cells/sec with >90% sample purity and 90% cell viability after sorting. The sorting mechanism is realized by exciting dynamic vapor bubbles with focused laser pulses in a microfluidic PDMS channel. The explosive bubble expansion generates fast fluid flows which...
In this paper, the dual-view display used micro-prism array splitter we designed is initially fabricated by using the LIGA-like technology. The roughness of the prism surface is less than 1 nm.
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