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Current transgenic animal models of Hirschsprung disease are restricted by limited survival and need for special dietary care. We used small animal colonoscopy to produce chemically ablated enteric nervous system in the distal colon and rectum of normal mice.Adult C57BL/6 mice underwent colonoscopy with submucosal injection of 75–100 μL of saline (n = 2) or 0.002% (n = 2), 0.02% (n = 15), or 0.2%...
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