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As the real sample diagnosis using resonance-based microcantilevers, a simple method is strongly suggested that capture probe immobilized on the cantilever directly hybridize with nanoparticles conjugated detection probe. Two different dimensions of microfabricated piezoelectric cantilevers and two different sizes of nanoparticles were used in this experiment. As a result, the most suitable conditions,...
We report the nanomechanical microcantilevers operated in vibration modes (oscillation) with use of RNA aptamers as receptor molecules for label-free detection of hepatitis C virus (HCV) helicase. The nanomechanical detection principle is that the ligand-receptor binding on the microcantilever surface induces the dynamic response change of microcantilevers. We implemented the label-free detection...
The sensitivity of immunoassays strongly depends on the binding affinity of the antibodies, which is also limited by a variety of other factors that are specific to the assay configuration, the type of label and instrument system used for detection. Magnetic beads are used in order to increase sensitivity in diagnosis system using the piezoelectric microcantilever. Use of magnetic beads is seemingly...
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