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After administration of 13C‐labeled glucose, the activity of the pentose phosphate pathway (PPP) is often assessed by the distribution of 13C in lactate. However, in some tissues, such as the well‐oxygenated heart, the concentration of lactate may be too low for convenient analysis by NMR. Here, we examined 13C‐labeled glutamate as an alternative biomarker of the PPP in the heart. Isolated rat hearts...
The source of hyperpolarized (HP) [13C]bicarbonate in the liver during metabolism of HP [1‐13C]pyruvate is uncertain and likely changes with physiology. Multiple processes including decarboxylation through pyruvate dehydrogenase or pyruvate carboxylase followed by subsequent decarboxylation via phosphoenolpyruvate carboxykinase (gluconeogenesis) could play a role. Here we tested which metabolic fate...
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