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A study was carried out to test the suitability of Applied Biosystems Quantifiler™ Human Quantification Kit and validate it for forensic casework. The Quantifiler™ assay was performed using an Applied Biosystems 7900HT Real-time PCR system. The validation exercise comprised five parts: (1) Reproducibility, (2) Sensitivity, (3) Effect of bacterial DNA, (4) Effect of reducing reaction volume, and (5)...
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