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Binding reaction of methylene blue (MB) with human serum albumin (USA) in the presence of sodium dodecyl sulfate (SDS) was studied. It was found that at pH = 2.87 positively charged MB bound firstly to HSA via hydrogen and hydrophobic bonds to form MB—HSA aggregate. The aggregate contained many positive charges and attracted negatively charged SDS to form macromolecules, which induced a great enhancement...
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