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Placental soluble fms-like tyrosine kinase 1 (sFlt1) release is increased in preeclampsia, which might be associated with increased shedding of Flt-1 through a disintegrin and metalloproteinase 10 (ADAM10). Here, we explored whether ADAM10 expression is regulated by hydrogen sulfide (H2S) in placenta. Pro- and active-ADAM10 expression were significantly increased in preeclamptic placentas and inversely...
Trophoblast cells play vital roles in the processes of embryonic implantation and placentation. Many toxicological compounds can induce the malfunction of trophoblast cells, resulting in implantation failure or early embryonic loss. The finite lifespan of primary trophoblast cells limits investigation of the long-term effects of some toxicological compounds on trophoblast cells in vitro. In this study,...
Fetal growth restriction (FGR) is a major cause of neonatal morbidity and mortality. There is evidence to show that FGR is associated with oxidative stress. Lipoxin A 4 (LXA 4 ) is an anti-inflammatory mediator and is considered to be a potent endogenous “stop signal” in inflammation. LXA 4 has been extensively studied preclinically in many diseases related to inflammation...
We report a method that allows for a 2–9-fold increase in transfection efficiency compared to the standard cationic lipid-based protocol. The method involves a brief incubation of freshly trypsinized cells with transfection complexes, followed by incubation in cell growth medium containing serum and antibiotics. The method is simple, cost-effective, and can be applied to both DNA and siRNA transfections...
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