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Transmission of active transcriptional states from mother to daughter cells has the potential to foster precision in the gene expression programs underlying development. Such transcriptional memory has been specifically proposed to promote rapid reactivation of complex gene expression profiles after successive mitoses in Drosophila development [1]. By monitoring transcription in living Drosophila...
The early Drosophila embryo is an ideal model to understand the transcriptional regulation of well-defined patterns of gene expression in a developing organism [1]. In this system, snapshots of transcription measurements obtained by RNA FISH on fixed samples [2, 3] cannot provide the temporal resolution needed to distinguish spatial heterogeneity [3] from inherent noise [4, 5]. Here, we used the MS2-MCP...
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