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Phospholipase A 2 (PLA 2 ) activity in the soluble fraction of rat kidney yielded three peaks on DEAE cellulose column chromatography. From these three, we purified two PLA 2 isoforms to near-homogeneity. Both had a molecular weight of approx. 14 000 on SDS-PAGE, and immunochemical and enzymological studies indicated that one is a 14 kDa type I PLA 2 and the other...
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