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Eine Schlüsseleigenschaft lichtaktivierbarer Markierungen für die ultrahochauflösende Bildgebung ist die Zahl der pro Molekül emittierten Photonen. In der Zuschrift auf S. 1755 ff. beschreiben L. D. Lavis et al. eine neue Markierung für die Lokalisierungsmikroskopie, photoaktivierbares Si‐Q‐Rhodamin, die bessere Photonenzahlen liefert und ausreichend rotverschoben für eine Mehrfarben‐Bildgebung emittiert...
The rhodamine system is a flexible framework for building small‐molecule fluorescent probes. Changing N‐substitution patterns and replacing the xanthene oxygen with a dimethylsilicon moiety can shift the absorption and fluorescence emission maxima of rhodamine dyes to longer wavelengths. Acylation of the rhodamine nitrogen atoms forces the molecule to adopt a nonfluorescent lactone form, providing...
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