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The vinculin binding site on α-actinin was determined by cryo-electron microscopy of 2D arrays formed on phospholipid monolayers doped with a nickel chelating lipid. Chicken smooth muscle α-actinin was cocrystallized with the β1-integrin cytoplasmic domain and a vinculin fragment containing residues 1–258 (vinculin D1 ). Vinculin D1 was located at a single site on α-actinin with 60–70%...
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