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An approach based on the polymerase chain reaction (PCR) was used to isolate additional members of the G-linked receptor family from a rat striatal λgtII cDNA library. Priming with one degenerate probe corresponding to highly conserved consensus sequences in the third transmembrane (TM) domain of 15 G-linked receptors and sequences in the phage vector resulted in one clone (G-13) encoding a dopamine...
The glycoprotein nature of the ligand binding subunit of photoaffinity-labeled striatal D 2 receptors was investigated. Upon photolysis, [ 125 I]N-azidophenethylspiperone covalently incorporated into a major band of M r , 94000 with an appropriate pharmacological profile for D 2 receptors as assessed by autoradiography following SDS-polyacrylamide gel electrophoresis...
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