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Using oligonucleotide primers designed to the known gene sequence of an (E)-β-farnesene (EβF) synthase, two cDNA sequences (MxpSS1 and MxpSS2) were cloned from a black peppermint (Mentha×piperita) plant. MxpSS1 encoded a protein with 96% overall amino acid sequence identity with the EβF synthase. Recombinant MxpSS1 produced in Escherichia coli, after removal of an N-terminal thioredoxin fusion, had...
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