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Thermophiles are microorganisms that inhabit at extremely high temperature environment. Thermoenzymes from thermophilic microorganisms are heat-stable, able to withstand denaturants of extremely acidic and alkaline conditions, and thus, have a considerable potential for many industrial applications. The aim of this study was to isolate and characterize amylolytic thermophiles. Two isolates of amylase...
This paper describes design and development of transduction, filtering, stabilisation and linearization stages of a potentiometric circuit to convert micro-signals from a PZR microcantilever biosensor into readable signals for human stress. The biosensing principle is based on immobilization of the bioreceptor to produce a biochemical reaction. The novel potentiometric circuit converts this biochemical...
Innovation of ISFET with electrochemical and silicon technology has the advantage of ease of integration with associated signal processing, simplicity, portability and potential on-site screening. ISFET sensor plays a critical role in biomedical instrumentation system. It serves at the front end of instruments in signal acquisition and conditioning circuit, interfacing between the electronic signals...
This paper presents a study on electrode for the amperometric detection of human stress based on salivary alpha amylase, emulated with different concentration of glucose, with the flow injection analysis biosensing system. Amperometric detection is an electrochemical voltammetric measurement approach, where the current intensity in a detection cell is regarded as a function of the concentration of...
This paper concerns the development of a potentiometric transduction circuit for a piezoresistive MEMS sensor to detect human stress. The biosensor is comprised of a bioreceptor and a transducer. The sensing principle is based on immobilization of the bioreceptor to produce a biochemical reaction. The novel piezoresistive microcantilever sensor integrated with a transduction circuit converts this...
Previous works on the effects of salivary alpha amylase in respond to various stressors report encouraging findings on it being a good indicator of stress. Ellestad protocol is a clinical procedure to screen for coronary artery disease by introducing exercise induced physical stress. If a salivary based biomarker profile in accordance to a stress test protocol could be established, the critical stress...
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