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In this research we eliminated chrysanthemum stunt viroid (CSVd) from a highly infected chrysanthemum cultivar using a newly established method. Piato is one of the most difficult cultivars in which to obtain CSVd-free plants by conventional methods. Leaf primordium-free shoot apical meristems (LP-free SAMs) of Piato plants were dissected and attached to CSVd-free chrysanthemum or cabbage root tips...
A new method to regenerate plants from leaf primordia-free shoot apical meristem domes (LP-free SAMs) was developed by establishing the meristem dome on the cut surface of root tips. Ten days after culture, the viable rate of LP-free SAMs of chrysanthemum Piato attached to chrysanthemum root tips was 40%. Shoot regeneration was not observed from LP-free SAMs without the root tips. When LP-free SAMs...
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