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B7‐H4, one of the immunoregulatory proteins, plays an inhibitory role by inhibiting T cell proliferation and cytokine production. Nevertheless, the significance of soluble B7‐H4 (sB7‐H4) in autoimmune diseases is unclear. In our study, we developed two novel mouse anti‐human B7‐H4 monoclonal antibodies (mAbs) (clones 8D4 and 7E1) with utilities for flow cytometry, immunoblotting and immunofluorescence...