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Development of biomarkers for predicting the occurrence of hepatitis E virus related‐acute liver failure (HEV‐ALF) is conducive to prevention and early intervention. Serum samples from 250 HEV‐ALF patients, 250 patients with acute hepatitis E (AHE) and 250 health controls (HCs) were collected. We assessed the predictive ability of extracellular vesicle (EV)‐derived argininosuccinate synthase 1 (ASS1)...