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To investigate whether membrane-bound hormone receptors in corpus luteum (CL) couple to one common adenylyl cyclase (AC) or whether each receptor is coupled to its own AC.Plasma membranes from rat CL were used to assess the coupling of hormone receptors to AC under conditions allowing full expression of the enzyme system. The response to hCG, prostaglandin E2α (PGE2), and isoproterenol were analyzed...
To develop a simplified polymerase chain reaction (PCR) protocol on single cells for the purpose of preimplantation genetic diagnosis. Also to evaluate a new thermal cycler, RoboCycler 40 (Stratagene, La Jolla, CA), for reducing the time to complete PCR amplification.PCR amplification without DNA purification or reamplification of a 149 base pair (bp) segment of the human Y chromosome was used as...
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