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•Relatively little is known about the timing of genetic and epigenetic forms of somaclonal variation arising from callus growth. We surveyed for both types of change in cocoa (Theobroma cacao) plants regenerated from calli of various ages, and also between tissues from the source trees.
•For genetic change, we used 15 single sequence repeat (SSR) markers from four source trees and from 233 regenerated...
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