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An efficient protocol of callus induction, plant regeneration and long-term maintenance of embryogenic cultures for manilagrass was developed. Callus induction and embryogenic callus formation were influenced by cytokinins and nodal positions. Murashige and Skoog (MS) medium with 2 mg l−1 2,4-dichlorophenoxyacetic acid (2,4-D), 0.02 mg l−1 kinetin (KT) or 6-benzyladenine (BA) gave the highest frequency...
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