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Nylon membranes are first hydrolyed with dilute HCl and then treated with chitosan before being used as the affinity carrier. Papain as a ligand has been immobilized on the activated membranes with glutaraldehyde as a crosslinking agent. The factors involving with the activity of immobilized papain, such as concentration of glutaraldehyde, pH, temperature, reaction time, and the amount of added papain...
The application of cellulases in various industries demands highly stable enzymes, able to perform at extreme pH values and temperatures. In this study, the cellulase, which was immobilized on the chitosan-coated magnetic nanoparticles modified with alpha-ketoglutaric acid (alpha-KA-CCMNPs), exhibited a broader pH range of high activity and the loss of the activity of immobilized cellulase was lower...
The degradation of ten trichothecene mycotoxins by aqueous ozone was monitored by liquid chromatography–ultraviolet–mass spectrometry (LC–UV–MS). Saturated aqueous ozone (∼25ppm) degraded these mycotoxins to materials that were not detected by UV or MS. At lower levels (∼0.25ppm) of aqueous ozone, intermediate products were observed. On the basis of UV and MS data, it is proposed that the degradation...
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