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The uptake of choline for the synthesis and release of acetylcholine was investigated in brain slices by dynamic positron autoradiography using [ 11 C]choline. Brain slices (330 μm) were incubated with [ 11 C]choline in oxygenated Krebs-Ringer medium at 34 o C and serial two-dimensional time-resolved images of the uptake and release of radioactivity were recorded...
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