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A method is reported for the preparation of novel electrode by layer-by-layer assembly of haemoglobin (Hb), gold (Au) nanoparticles, chitosan (CS) and graphene (GR) onto glassy carbon electrode (GCE). The Au/GR–CS substrate shows an obvious promotion for the direct electron transfer between Hb and GCE. The surface concentration (Г*) of Hb on the as-modified electrode can be as high as 4.33×10 ...
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