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11β-hydroxysteroid dehydrogenase (11-HSD) catalyzes the interconversion of corticosterone and 11-dehydrocorticosterone in rats, or cortisol and cortisone in humans. The liver or Type I isozyme is a widely distributed glycoprotein that utilizes NADP + as a co-factor. To study the role of glycosylation in maintaining enzymatic activity, we introduced mutations into the two potential...
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