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A new method is proposed for joint tracking of cell morphology and motion from 3D temporal cellular images. We adopt the framework of region-based active contours for segmentation, which is able to cope with objects having blurred boundaries. Motion estimation is performed by optical flow to increase the robustness and accuracy. Cell morphology and motion are modelled via a unified energy formulation...
Live imaging of neural stem cells and progenitors is important to follow the biology of these cells. Non-invasive imaging techniques, such as phase contrast microscopy, are preferred as neural stem cells are very sensitive to photoxic damage cause by excitation of fluorescent molecules. However, large illumination variations and weak foreground/background contrast make phase contrast images challenging...
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