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Insulin-like growth factor 1 (IGF-1) has many positive effects on neonates’ intestinal health. However, the production of IGF-1 involves complex and costly chromatography-based purification devices that greatly increase the cost of IGF-1. In this study, we applied the DAMP4 fusion technology to express IGF-1 in Bacillus subtilis cell factories without using these purification devices. After optimizing...
As a promising antibiotic candidate, it is necessary to find a safe method to produce the porcine cathelicidin antimicrobial peptide PR39. In this study, the antimicrobial peptide PR39 was successfully expressed with the maltose-inducible vector pGJ148 in the Bacillus subtilis WB800N. After induction with 3% maltose concentration, the fusion protein was successfully secreted onto the culture medium...
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