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A recombinant form of BACE1 (β-site amyloid precursor protein cleaving enzyme-1) corresponding to positions 46–454 of the extracellular domain of the original membrane enzyme was prepared. The recombinant BACE1 (rBACE1) had the kinetic parameters K m =5.5μM and k cat =1719s −1 . Using several libraries of substrates containing unnatural amino acids, the specificity of rBACE1...
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