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Clostridium sporogenes ATCC 25762 converted linoleic acid (c9,c12, 18:2) to c9,t11 conjugated linoleic acid (CLA, 18:2). The linoleic acid isomerase was membrane-associated and was very unstable, especially after being solubilized by detergents. Isomerase extraction, solubilization and stability were significantly improved by optimizing buffer composition and pH, and by minimizing detergent and protein...
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