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Gleichgesinntes Paar: Komplementäre Sonden mit zwei Pyren‐Markierungen wurden für die gezielte Vervielfältigung von DNA‐Sequenzen entworfen. Ohne die Zielsequenz liegen die Sonden in geschlossener Konformation vor, und die Pyren‐Einheiten sind räumlich getrennt. In Gegenwart der Zielsequenz läuft eine hybridisierende Kettenreaktion ab, die die Pyren‐Einheiten benachbarter Sonden zusammenführt, wodurch...
Make it a double: Complementary probes with two pyrene labels were engineered for the amplification of a target DNA sequence. In the stem‐closed conformation of the probes in the absence of the target, the two pyrene moieties were separated. The target propagated hybridization chain reactions to bring pyrene moieties on neighboring probes close together to form fluorescent pyrene excimers (see picture).
A one-dimensional addressable beads array for nucleic acids detection is presented in this paper. Molecular beacons (MBs) modified porous silica micro-beads were deposited along a micro-channel in sequence. This MB-functional beads array cooperating with microfluidic control allows multiple nucleic acid targets detection simultaneously with only once samples injection, a rapid dynamic hybridization...
Here some progresses of core-shell nanoparticles (NPs) for biomedical applications have been reported. A new synthesis mechanism for preparation of core-shell NPs by using water-in-oil (W/O) microemulsion technique has been investigated. And the applications of the core-shell NPs on cell recognition, oligonucleotide detection, single bacterial detection, pH nano-sensor for single cell, DNA recovery...
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