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According to the bias of codon utilization of Escherichia coli, a fragment encoding bovine lactoferricin(LfcinB) has been cloned and expressed in the Escherichia coli under the control of the T7 promoter. The target peptide was expressed by IPTG induction, purified by affinity chromatography with 6XHis tagged resin. The recombinant bovine lactoferricin appears to be successfully expressed, as it displays...
Soluble epoxide hydrolase (EPHX2) catalyses the degradation of the vasoactive and anti-inflammatory epoxyeicosatrienoic acids and may play a role in the pathophysiology of atherosclerosis. 1337 African-Americans and 1645 Whites from the CARDIA study were genotyped for 22 and 15 EPHX2 single nucleotide polymorphisms (SNPs), respectively, to examine the associations of common EPHX2 haplotypes and genotypes...
We performed a combination of single-variant- and haplotype-based analyses to investigate the association of peroxisome-proliferator-activated receptor γ (PPARγ) gene sequence variation with indices of adiposity, including body mass index (BMI) and waist girth, as well as insulin action, including fasting blood glucose and insulin levels. Nine polymorphisms, selected based on race-specific pairwise...
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