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The early onset oxidative damage within segments of the protein α-crystallin is examined by radical probe mass spectrometry by its treatment with reactive oxygen species under low-, moderate-, and high-oxidizing conditions. Five regions comprising the first 11 residues of the N-termini of the A and B subunits (A/B:1–11), central domains from each subunit B:57–69 and A:104–112, and a C-terminal segment...
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