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Assisted reproduction technologies (ART) require the reproductive quality of oocytes to be efficiently assessed. This chapter presents a cellular force measurement technique that allows for mechanical characterization of mouse oocytes during microinjection (i.e., in situ) without requiring a separate characterization process. The technique employs an elastic cell holding device and a sub-pixel computer...
Orientation control of biological cells under inverted microscopes is important for cell birefringent imaging and micromanipulation. Taking our microrobotic mouse embryo injection research as an example, this paper presents a cell orientation control system operated under inverted microscopes. A compact motorized rotational stage for inverted microscopy was developed for orienting the polar body of...
Taking a different architecture than manual operation and existing microrobotic systems, this paper presents the first automated system that employs novel microfabricated cell holding devices and vision-position based control of multiple motion control devices to achieve easy sample immobilization, rapid cell orientation, and fast injection of mouse embryos. The system requires minimal human involvement...
This paper presents the first demonstration of force-controlled micrograsping at the microNewton force level. The system manipulates highly deformable biomaterials (hydrogel microcapsules and biological cells) in an aqueous environment using a MEMS-based microgripper with integrated force feedback along two axes. The microgripper integrates an electrothermal V-beam microactuator and two capacitive...
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