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We sought to assess the effect of therapeutic hypothermia on the autophagy that occurred in ischemia-reperfused (IR) H9c2 cardiomyocytes.In control studies, the H9c2 cells at a density of 1×10 5 per culture dish in six-well plate were exposed to normoxic culture medium at 37°C for 12h. All assays contained appropriate controls and were performed in triplicate and repeated on three separately...
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