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Effects of electric pulses for activation on the development of bovine nuclear transfer embryos were examined in this study. Bovine oocytes from ovaries taken at slaughter were matured by the method previously reported (Shi et al, Theriogenology, 43:323). The oocytes were enucleated at 23 to 24 h after onset of IVM, and divided into two groups: 1) enucleated oocytes were electropulsed at 26 h by...
The objective of this study was to determine the optimum developmental stages of IVF bovine embryos for non-surgical transfer. Bovine oocytes recovered from ovaries taken at slaughter were matured and fertilized by the method previously reported (Shi et al, Theriogenology, 35: 271). At 22 to 24 h after insemination, oocytes were placed in co-culture with granulosa cell monolayers in a droplet of...
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