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Iron superoxide dismutases (FeSODs; FSDs) are primary antioxidant enzymes in Arabidopsis thaliana chloroplasts. The stromal FSD1 conferred the only detectable FeSOD activity, whereas the thylakoid membrane‐ and nucleoid‐co‐localized FSD2 and FSD3 double mutant showed arrested chloroplast development.FeSOD requires cofactor Fe for its activity, but its mechanism of activation is unclear. We used reversed‐phase...
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