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An enzyme‐free amperometric sensor based on a heptadecapeptide possessing an electroactive ferrocene (Fc) linker as ferrocene‐Gly‐Gly‐Gly‐Gly‐Phe‐Gly‐His‐Ile‐His‐Glu‐Gly‐Tyr‐Gly‐Gly‐Gly‐Gly‐Lys‐(CH2)4‐dithiocyclopentane self‐assembled on gold substrate was designed and fabricated for specific determination of L‐arginine (L‐Arg). The detection mechanism is based on conformational change of surface‐immobilized...