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A recombinant Bacillus subtilis strain (KN25) was generated for the large-scale preparation of catalase. The B. subtiliskatA gene encoding for catalase was cloned into the shuttle vector PRB374, downstream of the constitutively active vegII promoter, followed by transformation of the B. subtilis strain WB600 with the plasmid. The transformant strain, KN25 secretes high levels (3,500 U/ml) of catalase,...
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