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The extraordinary advancement in developing highly efficient packing materials for liquid chromatography (LC) has only partly touched chiral separations due, in part, to practical problems in synthesis and functionalization of sub-2-µm particles with chiral selectors, and, in part, to the lack of clear understanding of mass-transfer mechanisms in chiral chromatography. However, there is increasing...
Direct enantiomer separation of hypericin, pseudohypericin, and protohypericin was accomplished by high‐performance liquid chromatography (HPLC) using immobilized polysaccharide‐type chiral stationary phases (CSPs). Enantioselectivities up to 1.30 were obtained in the polar‐organic elution mode whereby for hypericin and pseudohypericin Chiralpak IC [chiral selector being cellulose tris(3,5‐dichlorophenylcarbamate)]...
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